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KMID : 0360220080490020340
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Journal of the Korean Ophthalmological Society
2008 Volume.49 No. 2 p.340 ~ p.351
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The Calretinin Immunoreactive Ganglion Cell Postsynaptic to the ON-Cholinergic Amacrine Cell in the Guinea Pig
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Kim Hyung-Chung
Suh Wool
Moon Jung-Il
Choi Kyu-Ryong
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Abstract
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Purpose : To demonstrate the characterization calretinin-immunoreactive displaced amacrine cells in the
ganglion cell layer using immunohistochemistry and electron microscopy.
Methods : For immunohistochemistry, sections from guinea pig retina were incubated with mouse monoclonal
antibody directed against calretinin. For double label studies, sections were incuated in mixture of mouse
monoclonal anti-calretinin or rabbit polyclonal anti-calretinin with following antibodies : goat polyclonal
anti-ChAT, rabbit polyclonal anti-GABA, mouse monoclonal anti-GABAA receptor α1, β2/3. Sections were
analyzed using Bio-rad Radiance Plus confocal scanning microscope. Stained sections from three guinea pig
were observed with transmission electron microscope.
Results : Calretinin immunoreactivity was present in displaced amacrine cells and ganglion cells gaving rise
to processes ramified in the inner part of the inner plexiform layer in stratum 4. The same stratum was also
occupied by the dendrites of ON-cholinergic amacrine cells. Double-labeling demonstrated that dendrites and
cell bodies of displaced amacrine cells colocalized with ON-cholinergic amacrine cells and dendrites of
ganglion cells directly overlapped with dendrites of ON-cholinergic amacrine cells. The synaptic connectivity
was identified by electron microscopy. Ganglion cell dendrites received synaptic input from ON-cholinergic
amacrine cell. GABAA receptor β2/3 subunit bands cofaciculates the dendrites of displaced amacrine cell and
ganglion cell that are juxtapose to the α1 subunit of GABAA receptor.
Conclusion : These results indicate that ON-cholinergic amacrine cells modulate calretinin-labeled ganglion
cell via GABAA receptor β2/3 in the guinea pig retina.
J Korean Ophthalmol Soc 49(2):340-351, 2008
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KEYWORD
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Amacrine cell, Calretinin, Ganglion cell, Immunohistochemistry
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